Novel Polymorphisms with in TLR4 Exon1 Sequences in Visceral Leishmaniasis and Pulmonary Tuberculosis Patients
Introduction: Leishmania and MycobacteriumTuberculosis share many similarities in their pathogenesis and both pathogens are macrophage parasites. The present study was carried out to determine the diversity of TLR4 gene in VL and pulmonary TB as innate immunity marker. Materials and Method: Confirmed VL patients, pulmonary TB patients and healthy individuals DNAs were analyzedfor TLR 4 exon1 mutationsafter stimulation with live Leishmania promastigotes and BCG. PCR based sequencing was done to determine the diversity of for toll-liked receptor 4 exon-1. Results: Three polymorphisms were detected for the first time in TLR4 exon1, TLR4-35C/T and TLR4-5C/T located in the transcription factor binding site, and TLR4+18C/T located in the coding region which resulted in the change from the amino-acid Threonine (polar) to Alanine (non-polar). Conclusion: The diversity in
TLR4 suggests possible variation in the innate immune responses of the two patients groups.