Variation in Growth Pattern and Morphological Appearance of Primary Monolayer Cultures of Chondrocytes and Neural Cells Isolated from the Chick Embryo at Different Stages

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Acta Medica International,2015,2,2,61-67.
Published:July 2015
Type:Original Article

Variation in Growth Pattern and Morphological Appearance of Primary Monolayer Cultures of Chondrocytes and Neural Cells Isolated from the Chick Embryo at Different Stages

Rupendra Shrestha, Asha Palat, Selvam Anbarasan, Solomon F. D. Paul*

Department of Human Genetics, Sri Ramachandra University, Chennai, India
†Authors have contributed equally in experimental work and manuscript preparation

Abstract:

Aim: This study was an attempt at investigating the variation in growth and morphology of primary avian chondrocyte and neural cell cultures established under standard laboratory conditions. Methods: For establishing chondrocyte cultures, the tibia were dissected from chick embryos that were 12 (E12), 14 (E14) and 15 (E15) days old. For the neural cell culture initiation, the two lobes of the forebrain were dissected from chick embryos that were 4 (E4), 8 (E8) and 12 (E12) days old. The final characterization of the cells
was performed by H&E and CFV staining. Results: In the chondrocyte cultures, two forms of morphology were observed which is reversible in process.The primary variation evaluated in chondrocytes cultures was the effect of the media on the state of the cells. Based on whether the cells were cultured in DMEM or MEM, there was a difference in the transformation of the attached cells from the differentiated to de-differentiated forms. The primary variation examined in neuron cultures was the embryonic age of the tissue and the effect that it had on the proliferation and neurite formation of the cells. E8 embryo neural cells cultures result in well-developed
cells with neurite formation along with axonal and dendritic out growths with highly complex interconnections between them. Conclusion: Ultimately, this study demonstrated the composition of culture media had an effect on the morphological appearance of the chondrocytes, as well as, confirmed that the culture of primary neurons is best performed using cells from an E8 embryo.

Single cell suspension after tissue mincing and digestion