HOXA9 Gene Expression and Cytogenetic Analysis in Egyptian Acute Myeloid Leukemia Patients

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Acta Medica International,2015,2,2,51-53.
Published:July 2015
Type:Original Article

HOXA9 Gene Expression and Cytogenetic Analysis in Egyptian Acute Myeloid Leukemia Patients

Hashem Neanaa1, Nahla A. M. Hamed1, Ahmad Raafat2, Iman Diab3, Ahmed Abdel Rahman1
 

1Department of Hematology, Faculty of Medicine, Faculty of Science, Alexandria University, Egypt,

2Department of Biochemistry, Faculty of Medicine, Faculty of Science, Alexandria University, Egypt,

3Department of Medical Biochemistry, Faculty of Medicine, Faculty of Science, Alexandria University, Egypt

Abstract:

Aim: we investigatedthe frequency of expression of HOXA9 gene in adult Egyptian acute myeloid leukemia (AML) patients and its relation to different cytogenetic abnormalities. Methods: 30 newly diagnosed AML patients (group 1) were the subject of our study. Ten healthy persons of matched age and sex were considered group ll (controls). Estimation of HOXA9 expression in AML blasts by RT-PCR was done to both groups. Results: Normal cytogenetic analysis was present in 20 cases (66.6%), t(15,17) in 3 cases, t(8,21) in 3 cases, 45 xy -7 in 1 case, t(16,16) in 1 case, 45 xy-20 in 1 case and 46 XX 1P+ in 1 case. NUP 98 -HOXA 9 gene was not detected in any of the studied case or in the control group. Conclusion: absence of HOXA9 gene in our randomly selected patientsmay be related  to its rarity in Egyptian population. However, further studies including larger population is still needed to confirm this finding with special stress on poor cytogenetic group.

Gel electrophoresis of RT-PCR