Isolation, Identification and Drug Resistance Testing of Mycobacterium tuberculosis by Recent Diagnostic Modalities at Teaching Hospital in Moradabad (UP)

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Acta Medica International,2016,3,2,51-55.
Published:September 2016
Type:Original Article

Isolation, Identification and Drug Resistance Testing of Mycobacterium tuberculosis by Recent Diagnostic Modalities at Teaching Hospital in Moradabad (UP)

Sudhir Singh¹, Umar Farooq², Sana Nudrat³

¹Assistant Professor, Department of Microbiology, TMMC&RC, Moradabad (U.P), India.

²Professor & Head, Department of Microbiology, TMMC&RC, Moradabad (U.P), India.

³PhD Scholar of Microbiology, TMMC&RC, Moradabad, (U.P), India.

Abstract:

Objective: Tuberculosis is the second leading cause of death in developing countries among all infectious diseases. Globally, drug resistance strain of Mycobacterium tuberculosis is a public threat. Due to diagnostic delay, inadequate infection control and poor drug supply there is a emergence of MDR- TB and XDR- TB. Our aim was to isolate and identify the drug resistant strain of M. tuberculosis by using newer diagnostic modalities.

Methods: Sputum sample and BAL fluid from 70 suspected cases were collected and analysed for Mycobacterium by Ziehl – Neelsen staining and liquid culture with molecular detection of drug resistant strain of M. tuberculosis.

Result: In our study, among the 70 patients 27 (38.5%) were positive for AFB by microscopy. On testing for Mycobacterium by BacT/Alert 3D system, 54 were found to be positive. On performing further identification and susceptibility of 54 isolates towards rifampicin and isoniazid by molecular method, 5 isolates (9.25%) were resistant to both rifampicin and isoniazid confirming as multidrug resistant. 5 isolates (9.25%) were sensitive to rifampicin and resistant to isoniazid and 2 isolates (3.70%) were resistant to rifampicin and sensitive to isoniazid. Whereas 5 isolates (9.25%) found to be negative for M. tuberculosis.

Conclusion: Our investigation highlights the importance of newer diagnostic modalities for isolation and identification of drug resistant strain of M. tuberculosis. Which ensure early and accurate diagnosis of patients with prevention of further transmission of disease.

PCR Hybridization strip