Detection of Metabolites of Vecuronium Bromide in Visceral Samples Solves A Typical Death Mystery- (Toxicokinetics- Studies Using Gc-Ms Technique)

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Acta Medica International,2015,2,1,125-133.
Published:January 2015
Type:Original Article

Detection of Metabolites of Vecuronium Bromide in Visceral Samples Solves A Typical Death Mystery- (Toxicokinetics- Studies Using Gc-Ms Technique)

Rakhi Khanna1*, R. S. Sharma2
 

1Assistant Director, Forensic Science Laboratory, Rajasthan,

2Retired Deputy Director, Forensic Science Laboratory, Rajasthan

Abstract:

Introduction: On Sudden death of a medical student (20 year old girl) of National Institute of Medical Science autopsy materials along with site of injection were sent to F.S.L. It was informed during investigation that the girl took tetanus vaccine before death. In crime scene investigation Forensic Team observed that it was a case of gross negligence of dispensing wrong injection. Material: Tests were performed on viscera material viz., liver, spleen, kidney, lungs, brain, skin and blood etc. Mystery of suspected death was solved when a new GC-MS application was designed to get the unknown drug and various fragments of extracted material were studied. Method: A new method was developed on gas-chromatography-mass spectrophotometer and TL.C using various solvent systems is explained. Results: Metabolite fragments of vecuronium bromide a muscle relaxant were surprisingly observed in Viscera material, Blood sample and Skin piece from Leftt cubital fossa from this young girl, whose death is questioned. Conclusion: Structural elucidations of fragments provide a new approach to toxicokinetics. The explanation of fragments obtained were structurally compared with other neuromuscular blocking groups like atracurium and succinyl choline The presence of bromide attached cholest-5-en-Br, hydroxylated cholest-5-en-ol as hydroxylated product, acetylated fragments as cholest-5-enacetate
and piperidone-2-one present in visceral samples indicates structural part obtained from vecuronium bromide drug. These metabolites studies makes the case studies highly informative. Beside this new method of extractions, TLC systems and colouring reagents are also explained.

The gas chromatogram of Liver, Spleen, Kidney having peak at Rt- 9.04gave mass fragmentation for cholest-5-en-ol good probability