Adipose-Derived Stem Cells: An Optimized Protocol for Isolation and Proliferation

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Acta Medica International,2016,3,1,116-121.
Published:January 2016
Type:Original Article

Adipose-Derived Stem Cells: An Optimized Protocol for Isolation and Proliferation

Mahdieh Ghiasi1, Reza Tabatabaei Qomi1, Naser Kalhor1, Mohsen Sheykhhasan1*

1Stem cell laboratory, Stem cell laboratory, The Academic Center for Education, Culture and Research (ACECR)- Qom Branch, shabnam avenue, isar square, Qom, Iran

Abstract:

Background and Aims: Current advances in the researches on the stem cells has opened new approaches for their apply in tissue engineering and clinical trials. The most common sources of stem cells are adult and embryonic stem cells. Due to ethical issues, embryonic stem cells use in research has been hotly debated. Unlike embryonic stem cells, adult stem cell have not ethical problem for clinical and research. The purpose of present study was to stem cells isolation and proliferation from the human dipose tissue.

Materials and Methods: In this study, stem cells were successfully isolated from human adipose tissue by digestion with type I collagenase enzymes. In summary, adipose tissue were digested by type I collagenase enzyme. Subsequently, the cell solution was centrifuged for mature adipocytes and debris elimination and obtained sedimentation was cultured in culture medium contains Dulbecco's Modified Eagle's Medium (DMEM) via 1% penicillin/streptomycin antibiotics and 10% fetal bovine serum (FBS) at 37°C, 5% CO2 and 95% humidity.

Results: in this study, ADSCs were successfully isolated and proliferated. Human ADSCs were able to divide in our culture mediums.

Conclusion: The results of the current study were suggesting that this adipose-derived stem cell isolation protocol provides an effective and improved method for isolation and proliferation of these cells in order to tissue engineering application.

Schematic image demonstration of successful storage of ADSCs in liquid nitrogen tank