Molecular Detection of Mycobacterium Tuberculosis Complex from Clinical Sputum Samples in Patients Attending Tertiary Care Centre in Uttar Pradesh Province of India

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Acta Medica International,2016,3,1,102-106.
Published:January 2016
Type:Original Article

Molecular Detection of Mycobacterium Tuberculosis Complex from Clinical Sputum Samples in Patients Attending Tertiary Care Centre in Uttar Pradesh Province of India

Abstract:

Background: In the world, tuberculosis ranks second after HIV of all infectious agents leading cause of mortality and morbidity due to bacterial infections with HIV taking the first spot. India holds the global burden of TB in one fifth with more than 350,000 deaths each year. Though pulmonary TB (PTB) cases, account for the vast majority of the total TB burden, almost 10-15 per cent of total cases are extra-pulmonary infection. Methods: Mycobacterium Tuberculosis complex were detected in 50 clinical sputum samples by using Polymerase chain Reaction (PCR) and BacT/Alert. Results: In our study, 50 sputum clinical samples were taken, out of which 11(22%) were smear positive & 39 (78%) were smear negative. Out of 11 smear positive 10 (90%) were MTB (Mycobacterium Tuberculosis) & 01 (10%) was NTM (Non-Tuberculous mycobacteria) and in 39 smear negative,15 (38.47%) were M. tuberculosis & 02 (5.12%) were NTM and 22 (56.41%) samples were negative by using PCR. By BacT/Alert 3D system, out of 50 clinical samples only 15(30%) samples were positive and 35 (70%) samples were negative for M. tuberculosis complex. Conclusions: It is concluded that result obtained from our study, Mycobacterium tuberculosis complex was detected by PCR from clinical samples has high specificity (99%) and sensitivity (95%) than BacT/Alert 3D system.

PCR Hybridization strip